| August 2025Aug 24 |  Serie A | Atalanta 1–1 PisaRegular Season - 1 | D |
| Aug 30 | Serie A | Pisa0–1 AS RomaRegular Season - 2 | L |
| September 2025Sep 14 | Serie A | Pisa0–1 UdineseRegular Season - 3 | L |
| Sep 22 | Serie A | Napoli 3–2PisaRegular Season - 4 | L |
| Sep 28 | Serie A | Pisa0–0 FiorentinaRegular Season - 5 | D |
| October 2025Oct 05 | Serie A | Bologna 4–0PisaRegular Season - 6 | L |
| Oct 18 | Serie A | Pisa0–0 Hellas VeronaRegular Season - 7 | D |
| Oct 24 | Serie A | AC Milan 2–2PisaRegular Season - 8 | D |
| Oct 30 | Serie A | Pisa0–0 LazioRegular Season - 9 | D |
| November 2025Nov 02 | Serie A | Torino 2–2PisaRegular Season - 10 | D |
| Nov 07 | Serie A | Pisa1–0 CremoneseRegular Season - 11 | W |
| Nov 24 | Serie A | Sassuolo 2–2PisaRegular Season - 12 | D |
| Nov 30 | Serie A | Pisa0–2 InterRegular Season - 13 | L |
| December 2025Dec 08 | Serie A | Pisa0–1 ParmaRegular Season - 14 | L |
| Dec 12 | Serie A | Lecce 1–0PisaRegular Season - 15 | L |
| Dec 21 | Serie A | Cagliari 2–2PisaRegular Season - 16 | D |
| Dec 27 | Serie A | Pisa0–2 JuventusRegular Season - 17 | L |
| January 2026Jan 03 | Serie A | Genoa 1–1PisaRegular Season - 18 | D |
| Jan 06 | Serie A | Pisa0–3 ComoRegular Season - 19 | L |
| Jan 10 | Serie A | Udinese2–2PisaRegular Season - 20 | D |
| Jan 16 | Serie A | Pisa1–1AtalantaRegular Season - 21 | D |
| Jan 23 | Serie A | Inter6–2PisaRegular Season - 22 | L |
| Jan 31 | Serie A | Pisa1–3SassuoloRegular Season - 23 | L |
| February 2026Feb 06 | Serie A | Hellas Verona0–0PisaRegular Season - 24 | D |
| Feb 13 | Serie A | Pisa1–2AC MilanRegular Season - 25 | L |
| Feb 23 | Serie A | Fiorentina1–0PisaRegular Season - 26 | L |
| March 2026Mar 02 | Serie A | Pisa0–1BolognaRegular Season - 27 | L |
| Mar 07 | Serie A | Juventus4–0PisaRegular Season - 28 | L |
| Mar 15 | Serie A | Pisa3–1CagliariRegular Season - 29 | W |
| Mar 22 | Serie A | Como5–0PisaRegular Season - 30 | L |
| April 2026Apr 05 | Serie A | Pisa0–1TorinoRegular Season - 31 | L |
| Apr 10 | Serie A | AS Roma3–0PisaRegular Season - 32 | L |
| Apr 19 | Serie A | Pisa1–2GenoaRegular Season - 33 | L |
| Apr 25 | Serie A | Parma1–0PisaRegular Season - 34 | L |
| May 2026May 01 | Serie A | Pisa1–2LecceRegular Season - 35 | L |
| May 10 | Serie A | Cremonese3–0PisaRegular Season - 36 | L |
| May 17 | Serie A | Pisa0–3NapoliRegular Season - 37 | L |
| May 23 | Serie A | Lazio2–1PisaRegular Season - 38 | L |
